Short and Scalable Synthesis of Plant‐Based Cholesterol in GMP Grade

A plant-based straightforward and short four-step synthesis of cholesterol starting from commercially available diosgenin is reported. This offers a large-scale production highly needed GMP-grade cholesterol, which part the lipid cocktail for assembly effective mRNA-based vaccines against COVID-19 pandemic. These lipids are formulated into delivery system mRNA fragments by forming Lipid Nano Particles (LNP) that introduce cells. Cholesterol plays crucial role because it stabilizes LNP facilitates endosome escape cytosol.